Detection of Haplosporidium pinnae from Pinna nobilis Faeces

Pinna nobilis (Linnaeus, 1758) is the largest bivalve endemic to the Mediterranean. It is distributed in a wide range of coastal environments, including estuaries. Pinna nobilis has recently become a critically endangered species (with almost 100% mortality) along the entire Spanish Mediterranean coast. This may be due to coinfections caused by Haplosporidium pinnae and bacterial pathogens such as Mycobacterium spp. We extensively sampled P. nobilis from Mar Menor lagoon (SE Spain), a site where individuals still survive. Using conventional PCR, we found Haplosporidium spp. in 7.1% of mantle and faecal DNA samples in different individuals of P. nobilis. We identified and quantified Haplosporidium pinnae in P. nobilis using Sanger sequencing and qPCR. Faecal H. pinnae detection is non-invasive, unlike biopsies. Therefore, this non-lethal and non-invasive sampling method could contribute to the welfare of living populations, particularly in eutrophic environments, where they are prone to septicaemia. The use of faecal DNA analysis could be a major advance in epidemiology and recovery assessment studies of P. nobilis.

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Resource type Text
Date of creation 2024-09-17
Date of last revision 2024-09-17
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Metadata identifier c84976b0-2e7e-5021-b094-fda67ddff83d
Metadata language Spanish
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ISO 19115 topic category biota
Other identifier DOI 10.3390/jmse10020276
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INSPIRE identifier ESPMITECOIEPNBMMENOR101
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Geographic identifier Murcia
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"{\"type\": \"Polygon\", \"coordinates\": [[[-2.34, 37.38], [-0.69, 37.38], [-0.69, 38.76], [-2.34, 38.76], [-2.34, 37.38]]]}"
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  1. Journal Marine Science and Engineering. Environmental Stressors and Pathology of Marine Molluscs
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Name of the dataset creator Lopez-Nunez, R., Cortes Melendreras, E., Gimenez Casalduero, F., Prado, P., Lopez-Moya, F. y Lopez-Llorca, L.V.
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